Composite

Part:BBa_K4836014:Design

Designed by: A Adithya Hitesh, M V P S Sriyanth, Sanchi Chaware, G Manonmani, Dhruvi Panchal, Akhshaya Mohan   Group: iGEM23_IISER-Tirupati-India   (2023-10-10)

CUW_0748 expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A T2A peptide sequence (BBa_K1537017) was used in between SERT and TagBFP. This produces two separate proteins instead of a fusion protein thus making sure that a fusion protein does not affect the functionality of SERT.


Source

The composite part contains multiple source basic parts

References